| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070077 |
| Chromosome: | chromosome 5 |
| Location: | 2373793 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238343 | outside_mRNA | ||
| Cre05.g800556 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGTGTCGTGACCTGGCGTGTGACCGTGCATATCGTGTGACTGAGTGA |
| Internal bar code: | GGCAATAAAATCTTGATATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3264 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAGGAGTTGACGTGGCA |
| Suggested primer 2: | ATACTGAGGCCCTGGTAGCA |