| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070082 |
| Chromosome: | chromosome 16 |
| Location: | 1144367 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650050 | (1 of 2) PTHR12231//PTHR12231:SF112 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 14, CHLOROPLASTIC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACAGCAGGGATACGTATGCGCACGAGCTTTGTATTCTTCCTCGCAGT |
| Internal bar code: | AAAAGACTAGTTCTAAGAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2063 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCGCCTGCTAACGCTAG |
| Suggested primer 2: | GTAGTGCTAGCAGCTACCGG |