Insertion junction: CLIP2.070094_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.070094
Chromosome:chromosome 6
Location:2728400
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g270950 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCGTACTGTGCATGGGTTTCGGCGGTGTGTCGGCGGCCGCTGTAGCG
Internal bar code:AATTCAGGGGTCTAGGTCGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:389
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACGTTCACTGCTGGGTGTC
Suggested primer 2:GTGTGCACAACAAGATGCGT