Insertion junction: CLIP2.070110_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.070110
Chromosome:chromosome 14
Location:2524355
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g624500 RRP46 Exosome complex exonuclease component; (1 of 1) K12590 - exosome complex component RRP46 (RRP46, EXOSC5) 3'UTR
Cre14.g624550 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCACGCACCCCTGGCACGCACACGCACCGGTCAAACGGGTGCGGTTA
Internal bar code:TCGTTAACCCAACCATCATGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:3572
LEAP-Seq percent confirming:76.2712
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTAGGACATGCAGTGGC
Suggested primer 2:GCACATACTCCCGCGATACA