| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070120 |
| Chromosome: | chromosome 7 |
| Location: | 5620937 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352300 | (1 of 3) 2.1.1.200 - tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase / TrMet(Xm32) | 3'UTR | |
| Cre07.g352350 | FHL7,FTSHI6,FHL3,FTSHi6 | (1 of 6) K03798 - cell division protease FtsH [EC:3.4.24.-] (ftsH, hflB); Chloroplast-import FtsH-like ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAGTGGGCCTGGTGTAAACACACGTGACACGGCCAACATCAAGCCCTT |
| Internal bar code: | TTGAATCGATGGCACCCACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1419 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTGATGGGTATACCGG |
| Suggested primer 2: | CTACACCACTGGCTTCGGTT |