| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070132 |
| Chromosome: | chromosome 7 |
| Location: | 3598238 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336850 | (1 of 3) PF01661 - Macro domain (Macro) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCATGAAGGTGGGGTTGGGCACGAAGCTTACGACACACAGGAGCTGGT |
| Internal bar code: | GGGGGTGCCCGTCGTAAGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5439 |
| LEAP-Seq percent confirming: | 99.3421 |
| LEAP-Seq n confirming: | 151 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTGCAATACGTGGGGTT |
| Suggested primer 2: | ATGACACCGCATGACACTGT |