| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.070148 |
| Chromosome: | chromosome 5 |
| Location: | 924262 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800526 | 5'UTR | ||
| lncRNA_TCONS_00046746 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGTAATGAATTCTTGAGGCAAGTTTGAGTATGGGGCGGCGAACTAGC |
| Internal bar code: | GCCCTCTCGGCTTCTTAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2687 |
| LEAP-Seq percent confirming: | 98.9583 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTTCGTAGCCACTGTTT |
| Suggested primer 2: | TGGTTCTCGAAATCCCTGGC |