| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070157 |
| Chromosome: | chromosome 15 |
| Location: | 830222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640400 | CLR4,OPR98,NCC2 | Nuclear Control of Chloroplast 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAGTAAGTCAGGGCCGCAGTGCCGCCCCTGGAGTTAAGGGCGATGGA |
| Internal bar code: | GGTTCTGAGTTTCTTTATACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5428 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTCTCCAATCTCCATCC |
| Suggested primer 2: | AGGTAACGATTGCTGCAGCT |