| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070163 |
| Chromosome: | chromosome 8 |
| Location: | 4400015 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385150 | 5'UTR | ||
| lncRNA_TCONS_00124269 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGTTTTCAACGAAGGTTTTGTTACGTCCTAGCATGTTATTATGGCAGA |
| Internal bar code: | GTGTAGCGCCAGTTTAGCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1730 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCCTTCGAAACCCTCCG |
| Suggested primer 2: | AGGGGAAATCAAGGAAGCGG |