| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070163 |
| Chromosome: | chromosome 8 |
| Location: | 4400030 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385150 | 5'UTR | ||
| lncRNA_TCONS_00124269 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGACGCAAACAACTCGCGCACGGCCCCCGCGCCAGCTCTTGTGGATAC |
| Internal bar code: | TTCGCATCCGTAATATGCTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 46.1538 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGGAAATCAAGGAAGCGG |
| Suggested primer 2: | TGTTCCTTCGAAACCCTCCG |