Insertion junction: CLIP2.070168_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.070168
Chromosome:chromosome 3
Location:781830
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g146167 TEF10a,HHL1 Homolog of Hypersensitive to High Light 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACTTTGCCAAACATATGTATCCTCTGCTCGATATGTCGTCCATGTTTG
Internal bar code:GTGTTCTCGCGATTACATATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4168
LEAP-Seq percent confirming:96.0526
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:76

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCGTAAATGTCCTGCTG
Suggested primer 2:GTTGTGACTTGTTCCGCACC