| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070170 |
| Chromosome: | chromosome 14 |
| Location: | 2934193 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627411 | (1 of 2) PTHR13146//PTHR13146:SF0 - UNCHARACTERIZED // SOLUTE CARRIER FAMILY 35 MEMBER F6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAAAGGAAACCACCCTCCCCCCAGTTGGCTACCGACCTTCCACAACC |
| Internal bar code: | TGTATACGTGTGGTGTTTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2995 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACCGTATCCAGGCAGCAG |
| Suggested primer 2: | GGGATGCCACGCTACTACTC |