Insertion junction: CLIP2.070180_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.070180
Chromosome:chromosome 5
Location:991997
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre05.g247150 (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTACTTGTCCGGTGTCCTACTCGGCGGTCCACCACAGCTCGAACGCTGA
Internal bar code:AGAACCTTCCTTGTGCCTCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:292
LEAP-Seq percent confirming:5.88235
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTTGACGCTGACGCTCAT
Suggested primer 2:GGCTACGGTGGGCTTAAAGT