| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070190 |
| Chromosome: | chromosome 16 |
| Location: | 3607014 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g672041 | (1 of 1) 2.1.1.228 - tRNA (guanine(37)-N(1))-methyltransferase / tRNA-(N(1)G37) methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCTGCAGGTTTTAACTATCAATTTCAATGCATGCGCTATGCAGCTTT |
| Internal bar code: | TATGCTCACGATACGTCGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 1.51515 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCCACACATCCACCCTG |
| Suggested primer 2: | TCCATACCAACAGCGCCATT |