| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070193 |
| Chromosome: | chromosome 11 |
| Location: | 3075582 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g475000 | IFT121,IFT122B,FAP118 | Intraflagellar Transport Protein 121; (1 of 1) PTHR16517:SF1 - WD REPEAT-CONTAINING PROTEIN 35 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCTCATCTTCTTGTGCCCCTAGCCTCGAGCTTCAGGTGAGCGATACC |
| Internal bar code: | GTAAGTCGCTTTCACTATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2417 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGTCCATCACACACGA |
| Suggested primer 2: | GGACCCCATGCCACAAAAAC |