| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070199 |
| Chromosome: | chromosome 11 |
| Location: | 1346448 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467710 | VSP1 | Hydroxyproline-rich cell wall glycoprotein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTTGCCTCTGTCCTTTGGCGCAGTTATTCCAGGCCTGCCTGCTTTCT |
| Internal bar code: | TAATGGGCGGAGCAGTAAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1339 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTGTATGGCTAGACAGG |
| Suggested primer 2: | CTTGTTGAAGCAGTTGCCCC |