| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070203 |
| Chromosome: | chromosome 1 |
| Location: | 3273224 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020950 | (1 of 1) K14852 - regulator of ribosome biosynthesis (RRS1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCCTGAGGGGTTACGGCGGCGGCATGTGTGCCGATGTGACGCCGGCG |
| Internal bar code: | AATGTGGCGCGTCCTTAGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCTGCAGTCTTCCTCTTC |
| Suggested primer 2: | GTGGGTTTGTGGGCGTTAAC |