| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070205 |
| Chromosome: | chromosome 16 |
| Location: | 5091890 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685600 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTCTACGCGCCTCCTCTTGTCATGCACGTCTATACCCTGTGAAGCTT |
| Internal bar code: | TGGGATGTGCGGAATTCTTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1380 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACGCGCATTGGTTGGAG |
| Suggested primer 2: | ATGACGCAAAGCAGGGGTTA |