| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070206 |
| Chromosome: | chromosome 17 |
| Location: | 3549360 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGCATAGCCGATAAAGGAGGGATACCACACCAAACTGTATGCATAAC |
| Internal bar code: | CTATAGCTTGGGGGCTGTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4977 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCACCTTGAGCTCAACT |
| Suggested primer 2: | GCCACACCTGCCGAAATTTT |