Insertion junction: CLIP2.070215_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.070215
Chromosome:chromosome 12
Location:4336736
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g519050 (1 of 9) IPR011008 - Dimeric alpha-beta barrel 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACTCTGTAGTTATCACTGCAAGGCTTGCATGTCGCCGTTGCGTCGTCT
Internal bar code:TTGGTGTTTTTTCGGACAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2009
LEAP-Seq percent confirming:97.7778
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:GTACCAGCTGGCTTGTAGCA
Suggested primer 2:GCACCAACTTGGATGCTTGG