| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070218 |
| Chromosome: | chromosome 9 |
| Location: | 2613231 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392250 | OTU5 | (1 of 2) IPR000104//IPR003323 - Antifreeze protein, type I // OTU domain; OTU-like cysteine protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTAATGAACTGCCCTCCCTTCACCCGCATCAAGAAGGGCTGCATGGC |
| Internal bar code: | TAATGTAATGATGTCGGGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2332 |
| LEAP-Seq percent confirming: | 98.6111 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATAGACATTGCGCGCGTA |
| Suggested primer 2: | CTGTTGACTGGTGTGACGGT |