| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070222 |
| Chromosome: | chromosome 6 |
| Location: | 1334483 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259100 | POB30 | Proteome of basal body 30 | 5'UTR |
| Cre06.g259150 | EFG8 | (1 of 1) PTHR23115//PTHR23115:SF155 - TRANSLATION FACTOR // SUBFAMILY NOT NAMED; Translation elongation factor Tu family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCAATAGAGAATGCTGATTCTAAAGCCAGAAAGACACGTAGTATGAA |
| Internal bar code: | GATAATACGGTGCTAATGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1344 |
| LEAP-Seq percent confirming: | 35.7143 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCAGTCCCCACATGAAC |
| Suggested primer 2: | TTCCCGTAACCTCGAAGCAC |