| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070223 |
| Chromosome: | chromosome 7 |
| Location: | 2480411 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329400 | SMM26 | (1 of 89) 3.6.4.13 - RNA helicase; S-adenosyl-L-methionine-dependent methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATAAAGCGGTAGCGCGCATGCTCGGCCAAAACCCCAGGCCTTAAGTT |
| Internal bar code: | ATAAGTGGCGACTTTGTGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1868 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACGGTGCCAGTGAAGTG |
| Suggested primer 2: | GGCCTAACATGAACCCGACA |