| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070230 |
| Chromosome: | plastome |
| Location: | 193224 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802334 | orf528 | unknown ORF | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCCGACTAATTTTGTTGTCAGAGTTTGGGTTAAAGGGTTTTTGAAAA |
| Internal bar code: | CTCCAGGGATTCTGACTGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 134 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCTCCCACCTTCGTTG |
| Suggested primer 2: | TGTGGTTAAGCTCGAACCCC |