| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070255 |
| Chromosome: | chromosome 6 |
| Location: | 1528724 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260800 | (1 of 1) K05755 - actin related protein 2/3 complex, subunit 4 (ARPC4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTTGCGCTGATGGCGGGAGCGCGAGAACTTGCCTGGCACGGGAATGT |
| Internal bar code: | GATAGCAACAGTTGTCCAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1410 |
| LEAP-Seq percent confirming: | 46.4286 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATCCAAGCACCAGGAAT |
| Suggested primer 2: | TGGTACTTGGATGCAGCTGG |