| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070275 |
| Chromosome: | chromosome 12 |
| Location: | 5779954 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532700 | (1 of 1) IPR000104//IPR007308 - Antifreeze protein, type I // Protein of unknown function DUF408 | 3'UTR | |
| Cre12.g532750 | EEP8 | (1 of 1) K10772 - AP endonuclease 2 (APEX2); Endonuclease/exonuclease/phosphatase family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACCACGTCCACCCACTCGGTTATCAAGTCAACTTGCATCTGTCCAG |
| Internal bar code: | TTCGTCCTGGTTCCGCTGCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 52.1739 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCAGATGTTGTTTGCGG |
| Suggested primer 2: | GGCTGCCAACGATGTGTTTT |