| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070279 |
| Chromosome: | chromosome 2 |
| Location: | 3204151 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096850 | (1 of 1) PF05620 - Protein of unknown function (DUF788) (DUF788) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATAGAGCAGCTCCCCGGACTCCGAGTACTCGGGCTCCGCAAACCGCG |
| Internal bar code: | CCGTCTTCTCACAACACTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 2.22222 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTACGAACAGCGGCAAGA |
| Suggested primer 2: | TTGAGCCTCGTACTTCGCTC |