Insertion junction: CLIP2.070283_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.070283
Chromosome:chromosome 8
Location:1486111
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre08.g364800 (1 of 1) 6.3.5.3 - Phosphoribosylformylglycinamidine synthase / Phosphoribosylformylglycinamidine synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACATGCACACTCACCCACAACCACACCGCTGCGCCCCCGCACTC
Internal bar code:CTATAATTCCTTTTAGCACAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:97
LEAP-Seq percent confirming:56.25
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCCCTCCCCCTTCCTTA
Suggested primer 2:TTCTCCACATGAGCGGCTTT