| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070284 |
| Chromosome: | contig 52 |
| Location: | 12674 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre52.g802240 | (1 of 3) PF09763 - Exocyst complex component Sec3 (Sec3_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATTCAACAACTCCCTCCTCCTTGAACGACTACCCCTCCATTCCTCA |
| Internal bar code: | CCTAAAGGGTTAAAGTCGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 156 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTATGAGGGTGTGGGG |
| Suggested primer 2: | AACGTCGTGCGGTATGAGTT |