| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070290 |
| Chromosome: | chromosome 17 |
| Location: | 5566178 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g739600 | (1 of 1) PF03988 - Repeat of Unknown Function (DUF347) (DUF347) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAACGATCACTGCAATCTGGTAGGTGTCCAGTAAGCAAGGTTCGTTG |
| Internal bar code: | ATACGTAGGTGTGATCTATACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1271 |
| LEAP-Seq percent confirming: | 32.7586 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGATGCAGTCCACTTCCT |
| Suggested primer 2: | TCAGCTTCATTCACGCAGGT |