| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.070292 |
| Chromosome: | chromosome 9 |
| Location: | 2286069 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394700 | RNZ1 | (1 of 6) 3.1.26.11 - Ribonuclease Z / tRNase Z; Ribonuclease Z | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCACCGCCTCCTCCACGAACCACGGTTCTGGCCCTTTGTGCGAAGTC |
| Internal bar code: | AATCTGGTGCTCGACATCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTTGTATGCGGCCTAGG |
| Suggested primer 2: | CACCTTCCAAGCGGTGTAGT |