| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070295 |
| Chromosome: | chromosome 10 |
| Location: | 4488873 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451000 | TRR1 | (1 of 1) K15407 - queuine tRNA-ribosyltransferase subunit QTRTD1 [EC:2.4.2.29] (QTRTD1); Queuine tRNA-ribosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGCCTGCTGCTGACCTGGCCTCGCCCCTCGCACAACCTTAGCTAATAA |
| Internal bar code: | TCGTCCATGCAAGATTTCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 65 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGGAAGGTGGAAGTACC |
| Suggested primer 2: | CCCCTATCTGCCTATGCTGC |