| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070307 |
| Chromosome: | chromosome 2 |
| Location: | 1265060 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082000 | AGE1 | DNA repair glycosylase; (1 of 1) K03575 - A/G-specific adenine glycosylase (mutY) | 5'UTR |
| Cre02.g082050 | (1 of 1) K13115 - coiled-coil domain-containing protein 130 (CCDC130) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACACATAACAAAACAATCAACCTCCCTGCAGAAGGAGAAGCACTTCG |
| Internal bar code: | GACCTGGGATAGTTTATGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1468 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATACCCTGGTCCAGCTT |
| Suggested primer 2: | TCGAACAAGGAAGAAGCCCC |