| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070316 |
| Chromosome: | chromosome 6 |
| Location: | 2932125 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273250 | GPA2,GPAT2 | Glycerol-3-phosphate acyltransferase; (1 of 1) K13506 - glycerol-3-phosphate O-acyltransferase 3/4 (GPAT3_4, AGPAT9, AGPAT6) | 3'UTR |
| Cre06.g273300 | TST1 | (1 of 2) 6.1.1.3 - Threonine--tRNA ligase / Threonyl-tRNA synthetase; Threonyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGAAGGATAGGCGCGCGCGAGTGCTGTACAGGGGTGCGTGCTGGTGA |
| Internal bar code: | GGCTAAGGTGCCCAAGATATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2903 |
| LEAP-Seq percent confirming: | 96.5909 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGGTCGGATGGGAATCT |
| Suggested primer 2: | ATCAGGAGGACGAGGACGAA |