| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070327 |
| Chromosome: | chromosome 2 |
| Location: | 5659008 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115850 | (1 of 3) PF01987 - Mitochondrial biogenesis AIM24 (AIM24) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACTCATCAACGCGTGGCTGTAGCCACTTCGCTGCACAAGCCTACCCA |
| Internal bar code: | GATAGTGCGTGCTCAAGTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3361 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTCTAAACCACTGGACT |
| Suggested primer 2: | TTGACAGTGAGCCACGTCTC |