| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070335 |
| Chromosome: | chromosome 3 |
| Location: | 716422 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145907 | (1 of 1) 2.1.1.205 - tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGACAAATTCAACAGTCTTTCATACATGGATGTGCACCACGTCTTGCA |
| Internal bar code: | CTTTGTAGAACTGGTTTTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCAGGTGATCCCAGA |
| Suggested primer 2: | TGTGAACCAAGACATCGCCA |