| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070353 |
| Chromosome: | chromosome 3 |
| Location: | 8066754 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201332 | (1 of 11) 2.1.1.259 - [Fructose-bisphosphate aldolase]-lysine N-methyltransferase / (dimerizing)]-lysine 6-N-methyltransferase | 5'UTR | |
| Cre03.g201350 | (1 of 100) IPR001810 - F-box domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGAATGCAGCGCTGAATGCAGCGGTCCGAGCACGAGCTTATGGTTCT |
| Internal bar code: | TAACTAAAGCAAACATCTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2993 |
| LEAP-Seq percent confirming: | 38.4 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 125 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTTGTATGGCAGGTGGC |
| Suggested primer 2: | ATGTCGGCCACATTGTGACT |