| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070359 |
| Chromosome: | chromosome 2 |
| Location: | 6494129 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388800 | GDH1 | Glutamate dehydrogenase; (1 of 2) 1.4.1.3 - Glutamate dehydrogenase (NAD(P)(+)) / Glutamic dehydrogenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACAAGACCCGGCTCAGTCCGTCTCACCATGCATGCACAGGCACGTACC |
| Internal bar code: | GTGCTTCTCGACAAGCTAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 55.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCTGAGGGTCGAAGTCC |
| Suggested primer 2: | CTGTATGGCGCTACGGTGAT |