Insertion junction: CLIP2.070371_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.070371
Chromosome:chromosome 2
Location:4893101
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g109750 3'UTR
Cre02.g109800 CPLD32,FAO3 conserved FAD dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF184 - FAD NAD BINDING OXIDOREDUCTASES // FAD-DEPENDENT OXIDOREDUCTASE-LIKE PROTEIN outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGTAAGGCCAGCCCTGCCCTCATACTACCCAGGTCCCAGGGGAACCC
Internal bar code:CAGGATATGTCATCGCACTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3789
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTACGAGAGGTGGAATG
Suggested primer 2:AGGGTCAATAGTGGTGCTGC