| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070389 |
| Chromosome: | chromosome 6 |
| Location: | 5734848 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288500 | RRA2 | (1 of 3) PTHR10994//PTHR10994:SF3 - RETICULON // ARABINOSYLTRANSFERASE; Reduced residual arabinose 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCTCGTGTCGGAGTCTGCAGCCAGAGTCAACGTTCCGGCACGAACGC |
| Internal bar code: | GAGTAGGCCCGTTCCAAGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3056 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGTCATCAAGTGGTACA |
| Suggested primer 2: | CACGCCCCAATTTTTCCCAG |