| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070393 |
| Chromosome: | chromosome 8 |
| Location: | 254680 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358563 | (1 of 1) PTHR18934:SF85 - FI05376P | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGACTTCAAGCTGATCGTCACCTCCGCCACCCTGGACGCCGAGAAGTT |
| Internal bar code: | GGTGCTCGATGCGATTAGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGCGCTCGTACAGGATC |
| Suggested primer 2: | GAGCCAGTACTCGGTCATCG |