Insertion junction: CLIP2.070397_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.070397
Chromosome:chromosome 6
Location:1795596
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g263289 (1 of 1) 2.5.1.87 - Ditrans,polycis-polyprenyl diphosphate synthase ((2E,6E)-farnesyl diphosphate specific) / Rer2p Z-prenyltransferase 5'UTR
Cre06.g263300 (1 of 2) PF05648 - Peroxisomal biogenesis factor 11 (PEX11) (PEX11) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAAACGTGCGCTACAAGAAACAGCGCAAACCCAGTGCGTCCTCGCAGC
Internal bar code:CTGGGAAGCACGAAAAAATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1063
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTAACACTCAGGTGGCGT
Suggested primer 2:CTTGTGGACCTTGCTGTTGC