Insertion junction: CLIP2.070398_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.070398
Chromosome:chromosome 4
Location:3667676
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g228850 (1 of 107) PF00069//PF07714 - Protein kinase domain (Pkinase) // Protein tyrosine kinase (Pkinase_Tyr) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTACCTCAATGAAGCCGGGTTGACAAAGCTTACGTTTAATTTATGTCCC
Internal bar code:GTTTATACATCGTATGTTCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2704
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGACACACACACACACAC
Suggested primer 2:CCCGACCCAGTATTTGGTCC