| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070406 |
| Chromosome: | chromosome 3 |
| Location: | 122728 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143827 | FBB7 | Flagellar/basal body protein 7; (1 of 1) IPR001680//IPR011047//IPR011048//IPR017986 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // Cytochrome cd1-nitrite reductase-like, haem d1 domain // WD40-repeat-containing domain | 5'UTR |
| Cre03.g143847 | (1 of 3) PF07386 - Protein of unknown function (DUF1499) (DUF1499) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTACAGACACAGTCAGTAACAATCTTACGGTGCGAGCTGGCTAAGACG |
| Internal bar code: | CGGCGCGCGGTTTCTCTATCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3735 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCTGCGAGAACATGACA |
| Suggested primer 2: | ACATTGGGGAGGCAGTCATG |