| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.070408 |
| Chromosome: | chromosome 17 |
| Location: | 6609724 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g745497 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAAGTTTCAATGCGCTATGGCCTGCGCGCGCTAAGTGTTGCGGACGGA |
| Internal bar code: | GGTCACAAGCATGTGAAAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 913 |
| LEAP-Seq percent confirming: | 34.375 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGGGGCCATCAATGATT |
| Suggested primer 2: | AGCATCTGTGTCGCACTTGA |