Insertion junction: CLIP2.070411_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.070411
Chromosome:chromosome 3
Location:8726702
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g208497 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAGTCGTACTGTAAAGCTGTCTCCCTTTGGCGCCATTAGGCCGCTGACA
Internal bar code:TTTCTTGTAAATAACCACACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1687
LEAP-Seq percent confirming:7.40741
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTGGGAACGTGACAAGGC
Suggested primer 2:CCGCGGTTGTTGTTGAATGT