| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070415 |
| Chromosome: | chromosome 7 |
| Location: | 4317921 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341300 | XAP5 | (1 of 1) K13119 - protein FAM50 (FAM50, XAP5); transcription factor controlling ciliary gene expression | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATAGTACACGTATACTCTATGCCAGAAAGAAACCTCGCGAGGCCCGG |
| Internal bar code: | GCGGGGGCGGTTAATGCCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2621 |
| LEAP-Seq percent confirming: | 81.1321 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATGCTCTAGTGCTCGAT |
| Suggested primer 2: | CCCACCGTCTCGTTCTTGAA |