| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.070428 |
| Chromosome: | chromosome 6 |
| Location: | 1402487 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259650 | MPA7 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR10161 - TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5 | 3'UTR |
| Cre06.g259700 | (1 of 6) 3.1.2.2 - Palmitoyl-CoA hydrolase / Long-chain fatty-acyl-CoA hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGTAGGCGCTACGTAAGGCACGTATCCTAGGCCTCGTGCCCACGCGC |
| Internal bar code: | CTCATTGCGAGTTGGGTACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1087 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCGGCTGAGTCGTGATGC |
| Suggested primer 2: | ACCTTGTCAGGCTTCACGAG |