| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070456 |
| Chromosome: | chromosome 6 |
| Location: | 3321850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277550 | (1 of 3) 3.5.5.1 - Nitrilase | 3'UTR | |
| Cre06.g277600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCTTCAGATGTCTGCACAATGACCCTCCAATTATGGCGGCTGCGTT |
| Internal bar code: | CCTAAGAGGGCATATCCGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1637 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACGCGGGACCAATTCTCC |
| Suggested primer 2: | TGGTTGTGAGCAGCTAAGCA |