Insertion junction: CLIP2.070458_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.070458
Chromosome:chromosome 3
Location:642992
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145647 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGAGAGGGTGTGGCGAAGTAGTTTCTGGGGCCCTGTTGACGGCGAGGG
Internal bar code:TGCCGTCAGGAGGGCGATACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:4666
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTAACCCGCAGCTAACAA
Suggested primer 2:ATAATGCATGCGACGCAACC